Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to LH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled LH and unlabeled LH (Standards or samples) with the pre-coated antibody specific to LH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of LH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LH in the sample